Antigenic reactive regions of S-carboxymethylated .BETA.-lactoglobulin.

Interaction of S-carboxymethylated uteroglobin with progesterone.

S-Carboxymethylated uteroglobin, a small progesterone-binding globular protein, was studied by means of high-field proton magnetic resonance spectroscopy. Conformational changes induced by the steroid have been observed and indicate a well-defined rearrangement of the structure of the protein. The unusual stoichiometry of one progesterone to two uteroglobin dimers is confirmed by 1H NMR. His-8 ...

Development of Transgenic Mice Harboring Ovine Beta Lactoglobulin-Calcitonin Transgene

pH-induced structural transitions in beta-lactoglobulin.

Pharmaceutique units/mg; Novo, Copenhagen, Denmark) and a-chymotrypsin (Choay, Paris, France) were, under appropriate conditions, added to the partially purified enzyme in final concentrations of 50 and 250 pg/ml and incubated at 37°C for different time intervals, ranging from 1 to 24 h. In all proteinase-treated preparations the DPP IV activity measured exceeded 90% of the original DPP IV acti...

Comparison of immunomodulating properties of Beta-lactoglobulin and its hydrolysates.

Cow's milk allergy is one of the most common food allergies in childhood. Beta-lactoglobulin (β- lg) is a dominant allergen in cow's milk. Hydrolysis is known as an effective method to reduce the allergenicity of proteins. Thus, the objective of this study was to compare the allergenicity of β-lg and its hydrolysates using an animal model. Twenty four BALB/c mice were divided into three groups ...

Plasticizer effect on oxygen permeability of beta-lactoglobulin films.

Plasticizer effect on oxygen permeability (OP) of beta-lactoglobulin (beta-Lg) films was studied. Propylene glycol (PG), glycerol (Gly), sorbitol (Sor), sucrose (Suc), and polyethylene glycol at MW 200 and 400 (PEG 200 and PEG 400, respectively) were studied due to their differences in composition, shape, and size. Suc-plasticized beta-Lg films gave the best oxygen barrier (OP < 0.05 cm3 x micr...